Coding
Part:BBa_K4340601:Design
Designed by: Chein Yueh Liu Group: iGEM22_PuiChing_Macau (2022-09-29)
PYL8
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 305
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use the pET11a vector to design our PYL8 plasmid with T7 system.
Source
aatgcgatcaatcggttcggtgcgatcctgcaccgcaatgccttcgctcacatccgccaggcttttcaggttgcatttaatcagcgcttccacaaaatagcaggtttcatctttggtgttgccttccggcacatccaccacaaagctttcaatcaccagggtgcccgggcggccatcaataatttccggatgcaggctcataatgctgctatagttgcgcaggcgatgatcgccgccaataatgcgaatgctcagcagatgttcgttatcatccagcagttccaggcgttcggtgctggtggtcgccggcaggccgcttttcacatccacttcgcgcaggctgccaatttccaggttgccgcgcaccacgcagcggctcacaaacggtttatatttctgcggctgatcaaagcggcgcaccaggctccacacctgcggcaccggcgcgcgaatatgtttcaccagcgcgctgccgcactggttttcgcccggttcatggcgatgatggcggcgaatatattccatttccacgttgctcaggccgccgccgccatcgttgccaatgccgttcat