Coding

Part:BBa_K4340601:Design

Designed by: Chein Yueh Liu   Group: iGEM22_PuiChing_Macau   (2022-09-29)


PYL8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the pET11a vector to design our PYL8 plasmid with T7 system.


Source

aatgcgatcaatcggttcggtgcgatcctgcaccgcaatgccttcgctcacatccgccaggcttttcaggttgcatttaatcagcgcttccacaaaatagcaggtttcatctttggtgttgccttccggcacatccaccacaaagctttcaatcaccagggtgcccgggcggccatcaataatttccggatgcaggctcataatgctgctatagttgcgcaggcgatgatcgccgccaataatgcgaatgctcagcagatgttcgttatcatccagcagttccaggcgttcggtgctggtggtcgccggcaggccgcttttcacatccacttcgcgcaggctgccaatttccaggttgccgcgcaccacgcagcggctcacaaacggtttatatttctgcggctgatcaaagcggcgcaccaggctccacacctgcggcaccggcgcgcgaatatgtttcaccagcgcgctgccgcactggttttcgcccggttcatggcgatgatggcggcgaatatattccatttccacgttgctcaggccgccgccgccatcgttgccaatgccgttcat

References